MRSA Diagnostics

Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is antibiotic resistant staph infection that impacts many individuals in the United States. At the moment there is not perfect diagnostic tool for MRSA either turnaround time is large or the procedure is expensive. Therefor the goal of this project is to find a quicker cost effective diagnostic tool for MRSA screening. Develop a quick and cost effective method to detect if a patient has (MRSA) is the need statement for this project. When researching different diagnostic tools, it was determined microelectrical mechanics systems (MEMS) would be the best way to diagnosis MRSA due to the quick turnaround times and inexpensive test costs. The MEMS device we propose to create will use changes in impedance to determine if MRSA is present. This process works by immobilizing antibodies on the surfaces of gold electrodes which have a high specificity towards MRSA. Everything that is not MRSA will not bind to the antibodies and will flow out of the MEMS device. When MRSA does bind to the antibodies this will cause a change in impedance which we can measure with an impedance analyzer

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Key Terms

  • MRSA: Methicillin-resistant Staphylococcus aureus
  • MEMS: microelectrical mechanics systems
  • MSSA: Methicillin-suseptable Staphylococcus aureus
  • PCR: Polymerase chain reaction
  • Antigen or antibody
  • PDMS: Polydimethylsiloxane
  • Wet etching
  • Lithography
  • PR- Photoresist

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